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Deoxycholate effects on plasma solute clearance by gut perfusion in dog
E D Kwon1, H P Schedl, J F Donovan
1Department of Urology, University of Iowa, College of Medicine.
The Journal of Surgical Research
|November 1, 1994
Summary
Deoxycholate, a detergent, was studied for its effect on intestinal solute clearance in dogs. While it initially boosted clearances, steady-state results showed only modest increases for most solutes, with urea clearance decreasing.
Area of Science:
- Gastroenterology
- Nephrology
- Pharmacology
Background:
- Intestinal luminal perfusion is explored for uremia treatment.
- Deoxycholate's effect on solute clearance is debated, with conflicting prior study results.
Purpose of the Study:
- To systematically investigate deoxycholate's impact on plasma solute clearance in a canine jejunal Thiry-Vella loop model.
- To determine dose-dependent effects and steady-state clearance changes.
Main Methods:
- In vivo luminal perfusion of a 90-cm jejunal Thiry-Vella loop in conscious dogs.
- Perfusion with varying deoxycholate concentrations (2, 7, 15, 30 mM) and collection of sequential 30-min samples.
- Measurement of clearances for inorganic phosphorus, urate, urea, creatinine, sodium, potassium, calcium, and chloride.
Main Results:
- Deoxycholate increased clearances of most solutes, but steady-state increases were modest.
- Initial high clearances of inorganic phosphorus and urate were transient and concentration-dependent.
- Steady-state clearances of sodium, potassium, calcium, chloride, and creatinine increased with 2 mM deoxycholate.
- Urea clearance was decreased by deoxycholate.
Conclusions:
- Deoxycholate causes only modest increases in steady-state gastrointestinal solute clearance.
- Transient, non-steady-state effects may explain previous findings of marked clearance increases.
- Urea clearance is reduced by deoxycholate, suggesting complex interactions.