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[Changes in the phagocytic cell functions elicited by surgical intervention]
1First Department of Surgery, Hiroshima University, Japan.
Nihon Geka Gakkai Zasshi
|August 1, 1994
Summary
Surgical injury activates phagocytic cells like polymorphonuclear leukocytes (PMNs) and macrophages, enhancing their function. These cellular responses are key host defense mechanisms against surgical stress.
Area of Science:
- Immunology
- Surgical Pathology
Background:
- Phagocytic cells play a crucial role in host defense.
- The impact of surgical intervention on phagocytic cell function requires further elucidation.
Purpose of the Study:
- To investigate the functional changes in phagocytic cells following surgical intervention.
- To assess the role of these cellular changes in the host's response to surgical injury.
Main Methods:
- Analysis of superoxide production and Mac-1 expression in peripheral polymorphonuclear leukocytes (PMNs).
- Measurement of cytokine production (IL-1 beta, TNF alpha) in peripheral monocytes (Mo) and peritoneal macrophages (M phi).
- Evaluation of these parameters in 35 surgical patients.
Main Results:
- Increased superoxide production and Mac-1 expression in PMNs within 24 hours post-operation.
- Elevated IL-1 and TNF production by monocytes and peritoneal macrophages, peaking at 1 and 8 hours post-operation, respectively.
- Undetectable serum levels of IL-1 and TNF in all patients, suggesting localized cellular activation.
Conclusions:
- Surgical injury induces in vivo activation of local macrophages and circulating monocytes.
- Activated macrophages and monocytes likely prime PMNs through cytokine production (IL-1, TNF, G-CSF).
- Phagocytic cell activation represents a host defense mechanism against surgical injury.