A genetic marker of rabbit immunoglobulin VHa region: a109

A "new" rabbit immunoglobulin allotype belonging to the VHa series (a109) that seems particular to the Iberian O. cuniculus has been detected in a Portuguese wild rabbit population. This allotype was found in the wild population of Spain but not in those of France and Zembra island (Tunisia). Using antisera and monoclonal antibodies raised against already known VHa allotypes, we found that a 109 presents cross-reactivity with a1, a3, a100, a101, a107 and a108 allotypes. On the basis of studies of these cross-reactivities, we show that a109 is constituted of at least three variants (or subfamilies of variants) possessing determinants common with either a1 and a109, a3 and a109 or a1, a3 and a109 allotypes. These results are discussed according to recent data relative to generation of antibody diversity in rabbit.