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Testing prospective anticancer drugs against human tumors using simple in vivo models
V I Ryabkova1, A Lockshin, I G Strukov
1N.N. Blokhim Cancer Research Center, Russian Academy of Medical Sciences, Moscow.
Abstract:
The activities of established and experimental antitumor drugs were tested against the BRO and MeWo human melanomas transplanted in the subrenal capsule of phenotypically immunocompetent (CBA x C57BL/6)F1 mice. Immunosuppression was achieved with preliminary whole-body radiation, and cytological examination showed that the tumors of untreated mice consisted largely of viable tumor cells. Several drugs tested showed moderate activity against one or both tumors. The system described provides a basis for testing anticancer agents against a panel of human melanomas implanted in immunocompetent mice.
Insights
Researchers evaluated antitumor drugs against human melanomas in mice. This model offers a new way to test cancer drugs effectively in immunocompetent models.
Area of Science:
- Oncology
- Pharmacology
- Immunology
Background:
- Human melanoma xenografts are crucial for preclinical drug testing.
- Phenotypically immunocompetent mouse models offer a more relevant environment for evaluating drug efficacy.
Purpose of the Study:
- To assess the activity of established and experimental antitumor drugs against human melanoma xenografts.
- To establish a reliable preclinical testing system for anticancer agents using immunocompetent mice.
Main Methods:
- Human melanoma cell lines (BRO and MeWo) were transplanted into the subrenal capsule of (CBA x C57BL/6)F1 mice.
- Immunosuppression was induced via whole-body radiation prior to tumor implantation.
- Cytological examination confirmed tumor viability in untreated control groups.
Main Results:
- Several tested drugs demonstrated moderate antitumor activity against one or both melanoma models.
- The xenograft model in immunocompetent mice showed a high proportion of viable tumor cells.
Conclusions:
- The described subrenal capsule xenograft model in immunocompetent mice is a viable system for evaluating anticancer drug efficacy.
- This model provides a basis for testing a panel of anticancer agents against human melanomas.