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[Frequency of serotype G rotavirus isolated from children with diarrhea in Mérida, Yucatán, Mexico]
M R González-Losa1, M Puerto-Solís, G G Polanco-Marín
1Departamento de Virología del Centro de Investigaciones Regionales, Universidad Autónoma de Yucatán, Mérida.
Abstract:
During a period of six years (1985-1990), rotavirus G serotypes were investigated in 104 fecal samples isolates according to an immune enzyme assay using specific monoclonal antibodies against serotypes 1, 2, 3, and 4 of the VP7. The serotypes were established in 65 samples (62.5%) and could not be determined in 39 samples. In the 65 classified serotypes, 7 (6.7%) were found to belong to serotype 1, 23 (22.1%) to serotype 2, 12 (11.5%) to serotype 3, and 23 (22.1%) to serotype 4. The occurrence of the four serotypes during the six years was: serotype 3 was present in three of the six years; serotype 2 was detected in two epidemic outbreaks (1989 and 1990); serotype 3 appeared in the second year and was seen in the remainder of the study; and serotype 4 was present in the six years. We conclude that the four serotypes occur in our population and that future efforts to test the efficacy of any vaccine against this virus should evaluate a protective response against the four serotypes.
Insights
Rotavirus serotypes 1, 2, 3, and 4 were identified in fecal samples between 1985-1990. This study highlights the circulation of these four rotavirus G serotypes, crucial for vaccine development.
Area of Science:
- Virology
- Immunology
- Public Health
Context:
- Rotavirus is a leading cause of severe diarrheal disease in infants and young children globally.
- Understanding the circulating rotavirus G serotypes is essential for developing effective vaccines.
- This study investigated rotavirus G serotypes over a six-year period in a specific population.
Purpose:
- To determine the prevalence and distribution of rotavirus G serotypes (1, 2, 3, and 4) in fecal samples.
- To analyze the temporal occurrence of identified rotavirus serotypes during the study period (1985-1990).
Summary:
- An immune enzyme assay using monoclonal antibodies identified rotavirus G serotypes in 65 out of 104 fecal samples.
- Serotype 1 (6.7%), Serotype 2 (22.1%), Serotype 3 (11.5%), and Serotype 4 (22.1%) were detected.
- Serotype 4 was present throughout the six years, while Serotype 2 and 3 showed epidemic patterns.
Impact:
- The findings confirm the circulation of rotavirus G serotypes 1, 2, 3, and 4 in the studied population.
- This data is critical for informing the design and evaluation of rotavirus vaccines, ensuring broad protection against prevalent serotypes.