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Mating reaction in yeast protoplasts

A Svoboda

    Archives of Microbiology
    |November 2, 1976
    PubMed
    Summary

    Yeast protoplast conjugation requires cell wall regeneration. Wall-less protoplasts can activate mating in normal cells but cannot fuse until their cell walls reform, indicating cell wall integrity is crucial for Saccharomyces cerevisiae mating.

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    Area of Science:

    • Cell Biology
    • Mycology
    • Genetics

    Background:

    • Saccharomyces cerevisiae is a model organism for studying eukaryotic cell processes.
    • Cell wall regeneration is a critical step for yeast protoplasts to regain cellular functions.
    • Sexual reproduction in yeast involves specific mating types and cell-cell interactions.

    Purpose of the Study:

    • To investigate the conjugating ability of Saccharomyces cerevisiae protoplasts.
    • To determine the role of cell wall integrity in yeast mating.
    • To understand the process of sexual activation and fusion in yeast protoplasts.

    Main Methods:

    • Preparation of protoplasts from complementary haploid yeast strains.
    • Observation of agglutination and fusion in fresh and regenerating protoplasts.
    • Assessment of sexual activation in normal cells induced by protoplasts.
    • Analysis of conjugation frequency based on cell wall regeneration status.

    Main Results:

    • Fresh and growing protoplasts without complete cell walls did not show sex-specific agglutination or fusion.
    • Wall-less protoplasts could induce sexual activation in normal yeast cells of the opposite mating type.
    • Protoplasts regained the ability to conjugate with each other or with normal cells after regenerating their cell walls.
    • Conjugation frequency was low (around 1%) and directly correlated with the extent of cell wall regeneration.

    Conclusions:

    • Cell wall integrity is essential for initiating mating in Saccharomyces cerevisiae.
    • Sex-specific morphogenetic changes and conjugation occur only after protoplasts regenerate their cell walls.
    • Artificial agglutination cannot induce plasma membrane fusion in sex-activated protoplasts; cell wall contact is necessary.

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