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Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
Published on: July 18, 2019
Evaluation of a novel diagnostic test for canine parvovirus
D P Drane1, R C Hamilton, J C Cox
1Research and Development Division, CSL Limited, Parkville, Vic., Australia.
Abstract:
The CPV ELISA detection kit (CSL Ltd., Melbourne Australia) was developed as a rapid field test for the detection of canine parvovirus (CPV) antigen in canine faecal samples. The kit utilises a novel concept in enzyme immunoassay (ELISA) where the conjugate is lyophilised along with the solid-phase capture antibody in the test well. The CPV ELISA was compared to the haemagglutination assay (HA) test using electron microscopy (EM) and/or virus isolation (VI) to confirm infection. The CPV ELISA had a sensitivity of 87% and a specificity of 100% compared to 87% and 63% respectively for the HA. The poor specificity of the HA results in a low positive predictive value of 51% compared to 100% for the CPV ELISA. The CPV ELISA requires no specialised equipment, is simple to perform and provides a visible result in less than 15 minutes.
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