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An equine B cell surface antigen defined by a monoclonal antibody
C H Zhang1, W L Donaldson, D F Antczak
1Cornell Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
Veterinary Immunology and Immunopathology
|July 1, 1994
Summary
Researchers identified a novel equine B lymphocyte surface antigen using antibody WS 65. This marker is present on most B cells but absent on T cells and leukemic B cells, suggesting distinct characteristics from surface immunoglobulin.
Area of Science:
- Immunology
- Veterinary Medicine
- Cell Biology
Background:
- Equine B lymphocytes play a crucial role in the immune response.
- Characterizing cell surface markers is essential for understanding lymphocyte function and disease.
Purpose of the Study:
- To identify and characterize a novel surface antigen on equine B lymphocytes.
- To investigate the expression pattern of this antigen on normal and malignant equine lymphocytes.
Main Methods:
- Immunohistochemistry on frozen tissue sections using the WS 65 antibody.
- Immunoprecipitation of lymphocyte membrane molecules.
Main Results:
- The WS 65 antibody identified a surface antigen expressed on nearly all equine B cells, but not on T cells, granulocytes, or thymocytes.
- Immunohistochemistry showed strong staining in lymph node follicles and splenic nodules.
- The antigen was not detected on equine leukemic T cells or B cells, despite the presence of surface immunoglobulin on the latter.
- Immunoprecipitation yielded an 85-90 kDa band under reducing conditions.
Conclusions:
- The WS 65 antibody defines a unique equine B cell surface antigen.
- This antigen's molecular characteristics and lack of expression on malignant B cells differentiate it from surface immunoglobulin.
- This marker holds potential for B cell research and diagnostics in horses.