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Heat shock protein 65 immunoreactivity in experimentally induced polymorphic light eruption
J P McFadden1, P G Norris, R Cerio
1St. John's Institute of Dermatology, St. Thomas's Hospital, London, U.K.
Acta Dermato-Venereologica
|July 1, 1994
Summary
Polymorphic light eruption (PLE) involves increased heat shock protein 65 (HSP65) in skin cells after UV exposure. This suggests HSP65 may act as a photo-induced antigen triggering PLE lesions.
Area of Science:
- Immunodermatology
- Photodermatology
- Heat Shock Proteins
Background:
- Polymorphic light eruption (PLE) is a common photodermatosis.
- The role of photo-induced antigens in PLE pathogenesis is not fully understood.
Purpose of the Study:
- To investigate the expression of heat shock protein 65 (HSP65) in experimentally induced PLE lesions.
- To determine if HSP65 acts as a photo-induced antigen in PLE.
Main Methods:
- Skin biopsies were taken from experimentally induced PLE lesions in patients and controls.
- Immunohistochemistry using monoclonal antibody ML-30 was performed to detect HSP65.
- Minimal erythema dose determination and controlled UV irradiation were employed.
Main Results:
- PLE patients showed clinical inflammation and lesion development within 5 hours of irradiation.
- Increased HSP65 expression was observed in keratinocytes, endothelial cells, and dermal dendritic cells from 1-6 days post-irradiation.
- Normal subjects did not exhibit inflammation or increased HSP65 labeling.
Conclusions:
- HSP65 is upregulated in skin following UV irradiation in PLE patients.
- These findings support the hypothesis that HSP65 may function as a photo-induced antigen in the development of PLE.