Related Experiment Video
Updated: Aug 3, 2026

Strategies for Study of Neuroprotection from Cold-preconditioning
Published on: September 2, 2010
The endogenous ouabain-like sodium pump inhibitor in cold injury-induced brain edema
Z M Rap1, W Schoner, Z Czernicki
1Institute of Biochemistry and Endocrinology, Justus-Liebig-University of Giessen, Federal Republic of Germany.
Abstract:
An endogenous ouabain-like factor (EOLF) was measured in brain tissue of cats 12 and 24 hrs after cold injury-induced edema. EOLF was assayed via its inhibition of 86Rb+ uptake in human red blood cells in a fraction which was obtained from brain tissue by methanol extraction, chloroform treatment and purification of the water phase by C-18 HPLC. As compared to the contralateral hemispheres with an EOLF concentration of 605 +/- 71 pmol ouabain equivalents per g wet weight, the edematous hemisphere had significantly higher concentrations: 12 hours after cold injury it was 2600 +/- 1762 pmol (p < 0.03) and fell to 857 +/- 160 pmol ouabain equivalents/g wet weight after 24 hrs. Similar kinetics were evident for the EOLF concentrations in cerebrospinal fluid. It is suggested that the increase of EOLF in the edematous brain hemisphere may participate as a mediator in the development of vasogenic brain edema in the disturbance of the sodium metabolism.
More Related Videos
08:44Continuous IV Infusion is the Choice Treatment Route for Arginine-vasopressin Receptor Blocker Conivaptan in Mice to Study Stroke-evoked Brain Edema
Published on: September 1, 2016
05:57The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension
Published on: May 17, 2024
Related Concept Videos
Ischemic Stroke ll: Pathophysiology
Increased Intracranial Pressure l: Introduction
Increased Intracranial Pressure ll: Pathophysiology
Cerebral Edema l: Introduction
Cerebral Edema ll: Pathophysiology
Cytotoxic Edema: Pathophysiology