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Pseudorabies virus growth factor can be resolved into two active components

J Gasperík1, J Lesko, M Csabayová

  • 1Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava.

Acta Virologica
|April 1, 1994
PubMed
Summary
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Pseudorabies virus growth factor (PRGF) has two components that transform normal cells but lose their ability to repress transformed cells. Reconstituting both components restores the repression activity, implicating PRV glycoprotein gII.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Pseudorabies virus (PRV) produces a growth factor (PRGF) with dual activity: inducing cell transformation and repressing it.
  • PRGF's precise nature and mechanism of action require further elucidation.

Purpose of the Study:

  • To investigate the components responsible for PRGF's biological activities.
  • To explore the relationship between PRGF and PRV's gII glycoprotein.

Main Methods:

  • Partial purification and resolution of PRGF into distinct molecular weight components.
  • Assaying the transforming activity of PRGF components on MK-2 cells.
  • Assessing the transformation-repressing activity of PRGF components on Hela cells.
  • Neutralization assays using monoclonal antibodies against PRV gII glycoprotein.

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Main Results:

  • PRGF was resolved into two components (<300 kDa and <180 kDa).
  • Each component retained transforming activity in MK-2 cells but lost repression activity in Hela cells.
  • Simultaneous application of both components reconstituted the transformation-repressing activity.
  • Monoclonal antibodies against gII glycoprotein neutralized both PRGF activities.

Conclusions:

  • PRGF's dual activity is dependent on the interaction of its two distinct components.
  • The gII glycoprotein of PRV is likely involved in the synthesis or function of PRGF, supporting previous hypotheses.