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Transgenic models for study of pulmonary development and disease
S W Glasser1, T R Korfhagen, S E Wert
1Children's Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229-3039.
The American Journal of Physiology
|November 1, 1994
Summary
Transgenic mouse technology advances lung disease research. By studying gene function in vivo, scientists gain insights into lung development, morphogenesis, and disease pathogenesis, aiding in the creation of novel therapeutic strategies.
Area of Science:
- Pulmonary Medicine
- Developmental Biology
- Genetics
Background:
- Advances in molecular genetics enable gene isolation.
- Transgenic mouse technology bridges molecular biology and whole animal physiology.
- Assessing gene roles in vivo is crucial for understanding lung function.
Purpose of the Study:
- To review the application of transgenic mouse technology in studying lung development and disease.
- To highlight the use of gene targeting and insertion for creating disease models.
- To demonstrate the utility of transgenic mice in elucidating gene function in the respiratory system.
Main Methods:
- Utilizing lung epithelial cell promoter elements to drive gene expression in specific lung cells.
- Employing gene targeting in embryonic stem cells to mutate genes at precise chromosomal locations.
- Generating transgenic mouse lines through gene insertion and gene targeting for permanent inheritance.
Main Results:
- Elucidation of cis-acting elements controlling lung epithelial gene expression.
- Discernment of the roles of specific polypeptides in lung morphogenesis and tumorigenesis.
- Creation of animal models for pulmonary diseases, including cystic fibrosis.
Conclusions:
- Transgenic mouse technology is a powerful tool for studying lung development and disease.
- These models facilitate research into disease pathogenesis and the development of treatments.
- Permanent mouse lines provide valuable resources for ongoing pulmonary research.