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Spectrofluorometric method for NO determination

G Gabor¹, N Allon

¹Israel Institute for Biological Research.

Analytical Biochemistry

|July 1, 1994

Summary

This study introduces a new spectroscopic method for accurately measuring nitric oxide (NO) in solutions. The technique offers high sensitivity and a wide dynamic range, suitable for biological samples.

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Area of Science:

Analytical Chemistry
Biochemistry
Spectroscopy

Background:

Nitric oxide (NO) is a crucial signaling molecule in biological systems.
Accurate quantification of NO is essential for understanding its physiological and pathological roles.
Existing methods for NO detection may be limited by sensitivity, dynamic range, or susceptibility to interference.

Purpose of the Study:

To develop and validate a novel spectroscopic method for the quantitative determination of NO in aqueous solutions.
To establish the sensitivity and dynamic range of the proposed method.
To demonstrate the applicability of the method for biological samples and potential for in situ monitoring.

Main Methods:

Utilized ultraviolet (UV) absorbance of nitrosothiols.
Incorporated the inner filter effect for enhanced sensitivity.
Validated the method using aqueous solutions and brain homogenates.

Main Results:

Achieved a detection threshold of 5 x 10(-7) M for NO.
Established a wide dynamic range from 5 x 10(-7) M to 10(-4) M.
Demonstrated successful NO measurements in brain homogenates.

Conclusions:

The developed spectroscopic method provides a sensitive and reliable means for quantifying NO in aqueous solutions.
The method's robustness and immunity to electrical interference make it suitable for in situ monitoring applications.
Future applications include using optical fibers for real-time NO or glutathione monitoring in brain and blood vessels.

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