Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cardiac storage with UW solution and glucose

S E Fremes1, L R Guo, R D Furukawa

  • 1Division of Cardiovascular Surgery, University of Toronto, Ontario, Canada.

The Annals of Thoracic Surgery
|November 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Safety and efficacy analysis of a varicose vein sealant kit in the treatment of great saphenous vein dysfunction].

Zhonghua yi xue za zhi·2024
Same author

[Periodic dynamic observation and analysis of cellular and humoral immunity indexes of adults infected with Omicron BA.1].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024
Same author

RNA-sequencing reveals the metabolism regulation mechanism of sheep skeletal muscle under nutrition deprivation stress.

Animal : an international journal of animal bioscience·2021
Same author

Role of the active cycle of breathing technique combined with phonophoresis for the treatment of patients with chronic obstructive pulmonary disease (COPD): study protocol for a preliminary randomized controlled trial.

Trials·2021
Same author

Application of endoscopic sinus surgery in hemostasis for epistaxis.

Journal of biological regulators and homeostatic agents·2020
Same author

[Epidemiological investigation on a cluster epidemic of COVID-19 in a collective workplace in Tianjin].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2020
Same journal

A Health Tracking Application Leads to An Avid Cyclist Being Diagnosed with ALCAPA at 52 years old, Warranting a Takeuchi Repair.

The Annals of thoracic surgery·2026
Same journal

Impact of preoperative and postoperative modern guideline-directed medical therapy on survival following coronary artery bypass grafting.

The Annals of thoracic surgery·2026
Same journal

Does prior percutaneous coronary revascularization negatively affect the outcomes of subsequent coronary artery bypass grafting?

The Annals of thoracic surgery·2026
Same journal

Lymph Node Dissection and Chylothorax - Balancing Oncologic Benefit Against Morbidity.

The Annals of thoracic surgery·2026
Same journal

Preserved Antegrade Pulmonary Blood Flow in Bidirectional Glenn: Outcomes and Considerations for Staged Palliation.

The Annals of thoracic surgery·2026
Same journal

Domo Arigato, Mr. Roboto.

The Annals of thoracic surgery·2026
See all related articles

Adding glucose to University of Wisconsin solution (UWS) improves recovery of heart function and energy levels after cold storage. Supplementation enhances adenosine triphosphate levels and ventricular function, suggesting improved organ preservation.

Area of Science:

  • Cardiology
  • Organ Preservation
  • Biochemistry

Background:

  • Previous studies indicated glucose supplementation of University of Wisconsin solution (UWS) benefits cardiomyocyte recovery.
  • These findings were based on isolated human cardiomyocyte models.

Purpose of the Study:

  • To confirm the beneficial effects of glucose supplementation in UWS on isolated rodent hearts.
  • To evaluate the impact of varying glucose concentrations on heart function and energy recovery post-storage.

Main Methods:

  • Rodent hearts were stored in UWS with varying glucose concentrations (0, 10, 20, 30 mmol/L) for 8 hours at 0°C.
  • Hearts were assessed for adenine nucleotide levels via HPLC and ventricular function using a Langendorff apparatus with a left ventricular balloon.

Related Experiment Videos

Main Results:

  • Glucose supplementation increased post-storage and post-reperfusion adenosine triphosphate (ATP) levels compared to unmodified UWS.
  • A 10 mmol/L glucose concentration significantly improved developed pressure and coronary flow post-storage and reperfusion.
  • Higher glucose concentrations (20-30 mmol/L) also showed benefits in ATP levels.

Conclusions:

  • The addition of 10 mmol/L glucose to UWS enhances recovery of cardiac function and energy status after prolonged hypothermic storage.
  • These findings support previous in vitro results and suggest a practical application for improved organ preservation strategies.