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Identification of human seminal plasma components that bind IgG2 variant
Y K Jaiswal1, A L Kadam, S S Koide
1Center for Biomedical Research, Population Council, New York, New York 10021.
Archives of Andrology
|July 1, 1994
Summary
Human seminal plasma contains proteins that bind a specific IgG2 variant. These proteins and the IgG2 variant appear in 20% of individuals, suggesting their use as genetic markers.
Area of Science:
- Immunology
- Proteomics
- Human Genetics
Background:
- Human seminal plasma (hsp) contains soluble proteins.
- Some proteins in hsp can bind immunoglobulin (Ig) G.
Purpose of the Study:
- To identify and characterize hsp components that bind IgG.
- To investigate the specificity of this interaction with different IgG subclasses and variants.
Main Methods:
- Analysis of hsp components using molecular sizing.
- Testing binding interactions between hsp components and various human IgG subclasses and variants.
Main Results:
- Two novel hsp components (90 kD and 21 kD) specifically bind a variant of IgG2.
- This IgG2 variant is found in 20% of human sera; common IgG2 and other IgG subclasses do not bind.
- The 90-kD component is likely nonglycosylated, while the 21-kD component is glycosylated.
- Both hsp components and the IgG2 variant are present in approximately 20% of tested individuals.
Conclusions:
- A specific IgG2 variant interacts with distinct hsp proteins.
- The prevalence of these components (20%) suggests potential as genetic markers for population studies.