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Physiochemical and immunological characterization of hepatitis A virus nucleocapsids expressed in a vaccinia
1Shanghai Institute of Biochemistry, Academia Sinica, People's Republic of China.
Archives of Virology
|January 1, 1994
Abstract:
Expression of the entire open reading frame of hepatitis A virus was achieved in a vaccinia virus/T7/EMCV hybrid system. The expressed antigens were characterized as particles with buoyant densities and sedimentation coefficients typical of empty capsids and pentamers of HAV. This was further confirmed by electron microscopy. All capsid proteins were determined as components of the particles which raised neutralizing antibodies in an immunized rabbit.