[The identification of Bacillus cereus, Bacillus lichenformis and Bacillus subtilis strains using the coagglutination...

Polyclonal rabbit antisera against 4 B. cereus strains were selected from a total of 9 B. cereus antisera and pooled. This serum agglutinated all available B. cereus strains (n = 63) at a titre > or = 1:64 when tested by a slide co-agglutination reaction. One hundred and thirty-six Bacillus strains belonging to 17 other species reacted with higher serum concentrations only (titres mainly < or = 1:16). A pooled antiserum comprised of two B. licheniformis antisera and two B. subtilis antisera agglutinated all 43 B. licheniformis strains and all 38 B. subtilis strains at a titre > or = 1:128, but one strain each of the species B. pumilus, B. macerans and B. fastidiosus was also agglutinated at a titre 1:128.