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Position effect variegation in yeast

K D Tartof1

  • 1Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|October 1, 1994
PubMed
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Position effect variegation in yeast shows cell-cycle regulation. DNA replication influences chromatin, leading to gene silencing or expression, causing variegation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Position effect variegation (PEV) is a phenomenon where gene expression varies based on chromosomal location.
  • Traditionally studied in Drosophila, PEV occurs when euchromatic genes are near heterochromatin (centromeric or telomeric).
  • A similar variegation phenomenon has recently been identified in yeast.

Purpose of the Study:

  • To investigate the regulation of position effect variegation in yeast.
  • To elucidate the molecular mechanisms underlying yeast telomeric gene variegation.
  • To understand the role of the cell cycle in epigenetic gene regulation.

Main Methods:

  • Analysis of yeast telomeric gene expression.
  • Investigating the impact of DNA replication on chromatin structure.

Related Experiment Videos

  • Modeling the competition between silencing factors and transactivators during chromatin reassembly.

    • Main Results:

    • Yeast telomeric gene variegation is regulated by the cell cycle.
    • DNA replication triggers chromatin disassembly, creating a window for regulatory factor competition.
    • Chromatin reassembly after replication determines gene expression status (active or silent).

    Conclusions:

    • The cell cycle plays a critical role in epigenetic gene regulation through position effect variegation in yeast.
    • A model is proposed where DNA replication-mediated chromatin dynamics influence gene silencing.
    • This yeast system provides a valuable model for studying the mechanisms of variegation.