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Isolation of rat TATA factor as an active 75kDa protein complex
A Igarashi1, Y Makino, T Tamura
1Department of Biochemistry, Niigata University School of Dentistry, Japan.
Biochemical and Biophysical Research Communications
|November 15, 1994
Abstract:
A general transcription factor TFIID was purified from rat liver by a sequential column chromatography including organomercurial Sepharose and Sephacryl S-200 chromatographies, which were developed with an acidic buffer (pH5.5). Analyses by SDS-polyacrylamide gel electrophoresis, assay of eluants after renaturation, and immunoblotting showed that isolated TATA factor is an active 75kDa protein complex which contains 36kDa TATA-binding protein.