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Related Experiment Videos

Lipid peroxidation in open-heart surgery

G Valer1, G A Khoschsorur, S M Almdahl

  • 1Department of Surgery, University of Tromsø, Norway.

Perfusion
|January 1, 1994
PubMed
Summary

Cardiopulmonary bypass (CPB) increases malondialdehyde (MDA), a marker of lipid peroxidation, in arterial blood. This indicates oxidative stress during CPB, with no evidence of intracoronary MDA release during reperfusion.

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Area of Science:

  • Cardiovascular Surgery
  • Biochemistry
  • Oxidative Stress Research

Background:

  • Cardiopulmonary bypass (CPB) and myocardial ischemia-reperfusion injury are associated with oxygen free radical production.
  • Lipid peroxidation, indicated by malondialdehyde (MDA) levels, is a key marker of this oxidative damage.

Purpose of the Study:

  • To investigate the occurrence and extent of lipid peroxidation during CPB.
  • To measure malondialdehyde (MDA) levels in arterial and coronary sinus blood to assess oxidative stress.

Main Methods:

  • MDA levels were measured in arterial and coronary sinus blood samples from 13 patients undergoing cardiac surgery.
  • Measurements were taken pre-CPB, during CPB, and postoperatively.
  • Cold crystalloid cardioplegic arrest was used for myocardial protection.

Main Results:

  • Arterial MDA levels significantly increased during CPB, peaking 30 minutes after reperfusion (3.66 +/- 1.08 mumol/l).
  • Elevated MDA levels persisted for four hours post-CPB but normalized by the first postoperative day.
  • No significant difference was observed between arterial and coronary sinus MDA levels at any time point.

Conclusions:

  • CPB induces significant lipid peroxidation, evidenced by increased arterial MDA levels.
  • The study found no evidence of intracoronary release of MDA during reperfusion of the ischemic heart.
  • MDA measurement serves as a reliable indicator of systemic oxidative stress during CPB.

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