Gradient elution in micellar liquid chromatography. I. Micelle concentration gradient

Gradient elution in micellar liquid chromatography (MLC) is discussed. On the basis of the gradient elution theory, first developed by Snyder, equations were derived for the prediction of gradient retention times in micelle concentration gradient from isocratic data. Likewise, partition coefficients into micelles and stationary phase, and subsequently isocratic retention at different micelle concentrations can be estimated from two gradient runs. However, more studies need to be done to achieve better agreement between isocratic and gradient data. The equations will be useful for efficient development of practical separations by MLC.