Gradient elution in micellar liquid chromatography. II. Organic modifier gradients
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Updated: Oct 14, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
L S Madamba-Tan1, J K Strasters, M G Khaledi
1Department of Chemistry, North Carolina State University, Raleigh 27695-8204.
Abstract:
The application of organic modifier gradients in micellar liquid chromatography (MLC) is discussed. The equation derived by Snyder and co-workers describing gradient elution in hydro-organic reversed-phase LC was verified for organic solvent gradients in the presence of micelles. It is also demonstrated that the use of these gradients require little re-equilibration time due to the limited range of organic modifier concentration used in the gradient. This would result in shorter analysis time. Lastly, a practical application of the use of propanol and acetonitrile gradients in MLC is described.
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