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The effect of pulsed electromagnetic field (Diapulse) on cellular systems
M A Badea1, R Vasilco, D Sandru
1Interdisciplinary Research Group, Fundeni Hospital, Bucharest, Romania.
Abstract:
The effect of a 27.12 MHz pulsed electromagnetic field (Diapulse) on microbial growth is investigated. A strain of K 12 E. coli grown in complete Pennassay medium is subjected to Diapulse action for 30 min, at 8 hrs and 12 hrs of growth. In this experimental set-up, designed to be closed to the physiological conditions of open wounds, the Diapulse action does not promote any increase of cell population, indicating the safety of this type of therapy for wound healing process. The same K 12 E. coli strain grown in Pennassay medium for 2 hours is inoculated into a minimal growth medium and the lagless exponential growth thus obtained is followed by a spectrophotometric method. Diapulse field is applied to this lagless phase of cellular cultures at 30, 60, and 90 minutes after inoculation. A slight increase in the number of cells was observed at 2 and 4 hours after the Diapulse application, when the cultures were previously subjected to Diapulse action between the period of 60 and 90 minutes of their growth. A possible molecular mechanism for these effects is discussed.
Insights
Pulsed electromagnetic field (Diapulse) therapy shows no adverse effects on microbial growth in simulated wound conditions. However, Diapulse application during specific growth phases may slightly enhance bacterial proliferation.
Area of Science:
- Microbiology
- Biophysics
- Biotechnology
Background:
- Pulsed electromagnetic fields (PEMF) are explored for therapeutic applications.
- Understanding PEMF effects on microbial growth is crucial for wound healing therapies.
- The Diapulse device delivers 27.12 MHz pulsed electromagnetic fields.
Purpose of the Study:
- To investigate the impact of Diapulse on microbial growth under simulated physiological conditions.
- To determine if Diapulse therapy affects bacterial cell population.
- To explore potential growth-enhancing effects of Diapulse at specific growth stages.
Main Methods:
- Utilized Escherichia coli (E. coli) K 12 strain.
- Cultured bacteria in Pennassay and minimal growth media.
- Applied Diapulse (27.12 MHz PEMF) at different time points during growth.
- Monitored cell population using spectrophotometry.
Main Results:
- Diapulse application did not increase cell population in simulated wound conditions, suggesting safety.
- A slight increase in cell numbers was observed 2-4 hours post-Diapulse application when applied during specific lagless growth phases (60-90 minutes).
Conclusions:
- Diapulse therapy appears safe for wound healing applications concerning microbial growth.
- Specific timing of Diapulse application may influence bacterial growth dynamics.
- Further research into the molecular mechanisms underlying these observed effects is warranted.