Related Experiment Video
Updated: Aug 11, 2026

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
Published on: April 18, 2016
Glycotripeptides are released by yeast but not by mammalian microsomes
K van Leyen1, M Widemann, F Wieland
1Institut für Biochemie I, Heidelberg, Germany.
Abstract:
Glycotripeptides generated in vivo in the endoplasmic reticulum (ER) have been used as markers to assess the rate of vesicular bulk flow from the ER via the Golgi apparatus to the plasma membrane in mammalian cells. The applicability of such glycotripeptides as markers for bulk flow along this pathway has been questioned by a report on non-vesicular release of glycotripeptides from yeast semi-intact spheroplasts. We have therefore investigated direct release of glycotripeptides from yeast and from mammalian microsomes and report here that such release is specific to the yeast system and cannot be detected in mammalian microsomes.
More Related Videos
Related Concept Videos
Yeast Signaling
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Amino Acid Catabolism
Fungal Phylum Microsporidia
Inhibitors of Gram-positive Cell Wall Synthesis

