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Related Experiment Videos

Protein kinase C inhibitors decrease hyperalgesia and C-fiber hyperexcitability in the streptozotocin-diabetic rat

S C Ahlgren1, J D Levine

  • 1Department of Anatomy, University of California, San Francisco 94143-0452A.

Journal of Neurophysiology
|August 1, 1994
PubMed
Summary

Diabetic neuropathy causes pain hypersensitivity in rats. Protein kinase C inhibitors reduced this pain and nerve hyperexcitability, suggesting PKC activity contributes to diabetic neuropathy pain.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Diabetic Neuropathy Research

Background:

  • Streptozotocin-induced diabetes (STZ-D) in rats causes mechanical hyperalgesia.
  • C-fiber afferents in STZ-D rats exhibit hyperexcitability, not altered mechanical thresholds.
  • Protein kinase C (PKC) activity is implicated in C-fiber afferent excitability.

Purpose of the Study:

  • To investigate the role of protein kinase inhibitors in modulating mechanical hyperalgesia and C-fiber hyperexcitability in STZ-D rats.
  • To determine if inhibiting PKC activity can reverse the pain hypersensitivity associated with diabetic neuropathy.

Main Methods:

  • Utilized the Randall-Selitto paw-withdrawal device to assess behavioral mechanical nociceptive thresholds.
  • Administered intradermal injections of staurosporine and a PKC pseudosubstrate inhibitor peptide [PKC(19-36)].

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  • Recorded single C-fiber responses to standardized suprathreshold mechanical stimulation.

    • Main Results:

    • Intradermal protein kinase inhibitors dose-dependently increased mechanical nociceptive thresholds in STZ-D rats.
    • These inhibitors significantly reduced C-fiber hyperexcitability in STZ-D rats without affecting control rats.
    • Neither agent altered the baseline mechanical thresholds of C-fibers in either group.

    Conclusions:

    • Inhibition of protein kinase C activity effectively reduces mechanical hyperalgesia in a rat model of diabetic neuropathy.
    • C-fiber hyperexcitability, partly mediated by PKC, contributes to the observed hyperalgesia.
    • PKC inhibitors represent a potential therapeutic strategy for managing pain in diabetic neuropathy.