Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Analysis of the gene encoding human PC2, a prohormone processing enzyme]

S Ohagi1, H Yoshida, K Nanjo

  • 1First Department of Medicine, Wakayama University of Medical Science.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endothelium-dependent vasodilation is augmented by angiotensin converting enzyme inhibitors in healthy volunteers.

Journal of cardiovascular pharmacology·1992
Same author

Primary mediastinal diffuse large cell lymphoma initially presented with pericardial infiltration.

Internal medicine (Tokyo, Japan)·1992
Same author

Suppressive effect of prostaglandin E1, but not prostacyclin, on ouabain-induced ventricular fibrillation in guinea-pigs.

Fukushima journal of medical science·1992
Same author

A lymphocyte-specific protein tyrosine kinase, p56lck, regulates the PMA-induced internalization of CD4.

Biochimica et biophysica acta·1992
Same author

Contact hypersensitivity reaction to ovalbumin in newborn guinea pigs from maternally sensitized animals.

Journal of dermatological science·1992
Same author

Relationship between plasma atrial and brain natriuretic peptide concentration and hemodynamic parameters during percutaneous transvenous mitral valvulotomy in patients with mitral stenosis.

American heart journal·1992
Same journal

[Development of novel therapeutics for multiple myeloma and improvement of drug lag].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Clinical pharmacy services to patients of immunomodulatory drugs].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Therapeutic drug monitoring of the new anti-myeloma drugs in the treatment of multiple myeloma].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Prognostic value of minimal residual disease assessment using next-generation sequencing in multiple myeloma].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[The evaluation of minimal residual disease in multiple myeloma by an allele-specific oligonucleotide real-time PCR].

Nihon rinsho. Japanese journal of clinical medicine·2019
Same journal

[Evaluation of minimal residual disease in myeloma by multiparametric flow cytometry].

Nihon rinsho. Japanese journal of clinical medicine·2019
See all related articles

Prohormone Convertase 2 (PC2) gene expression was studied to understand insulin processing. Researchers analyzed the PC2 gene structure and promoter, identifying key regulatory regions essential for its activity.

Area of Science:

  • Molecular Biology
  • Genetics
  • Endocrinology

Background:

  • Prohormone Convertase 2 (PC2) is crucial for processing proinsulin into insulin.
  • PC2 expression is tissue-specific, primarily in pancreatic islets, pituitary, and brain.

Purpose of the Study:

  • To investigate the regulated expression of the human PC2 gene.
  • To characterize the structure and promoter region of the PC2 gene.

Main Methods:

  • Gene structure analysis and comparison with the human furin gene.
  • Localization of the hPC2 gene to chromosome 20p11.2.
  • Promoter activity analysis using deletion mutants and chrolamphenicol acetyltransferase assays.

Main Results:

  • The human PC2 gene spans over 130 kb and has 12 exons, with conserved exon-intron junctions compared to the furin gene.

Related Experiment Videos

  • The PC2 promoter is GC-rich, featuring Sp1 binding sites but lacking TATA or CAAT boxes.
  • A critical region from -1100 to -539 relative to the translation start site was identified as essential for PC2 promoter activity.

    • Conclusions:

    • The study elucidated the structural organization and promoter characteristics of the human PC2 gene.
    • Identified key regulatory elements within the PC2 promoter, providing insights into its tissue-specific expression and function in insulin processing.