Related Experiment Videos
Isolation and characterization of preantral follicles from foetal bovine ovaries
S C Hulshof1, J R Figueiredo, J F Beckers
1Department of Functional Morphology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
The Veterinary Quarterly
|July 1, 1994
Abstract:
A simple, mechanical method is described for the isolation of preantral follicles from bovine foetuses of 220-280 days of gestation. On average, 2918 + 621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter.