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Isolation, characterization and protection studies in mice of a streptomycin-dependent mutant of Streptococcus suis
N Foster1, J J Staats, M M Chengappa
1Department of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
Abstract:
An avirulent, streptomycin-dependent (Str-D) mutant of Streptococcus suis type 1/2 was produced and characterized by its antimicrobial susceptibility, growth kinetics, biochemical reactions and reversion rate. Homologous and heterologous vaccine trials in mice resulted in complete protection against challenge with S. suis types 1 and 1/2 and partial protection against challenge with S. suis type 2.
Insights
A new streptomycin-dependent mutant of Streptococcus suis was developed. This mutant showed promise as a vaccine, offering protection against S. suis types 1, 1/2, and 2 in mice.
Area of Science:
- Veterinary Microbiology
- Bacterial Genetics
- Vaccine Development
Background:
- Streptococcus suis is a significant pathogen in swine.
- Developing safe and effective vaccines against S. suis is crucial for animal health.
- Avirulent bacterial mutants are potential candidates for live attenuated vaccines.
Purpose of the Study:
- To create and characterize an avirulent mutant of Streptococcus suis type 1/2.
- To evaluate the immunoprotective potential of this mutant against homologous and heterologous S. suis serotypes.
Main Methods:
- Production of a streptomycin-dependent (Str-D) mutant of S. suis type 1/2.
- Characterization of the mutant's antimicrobial susceptibility, growth kinetics, biochemical profile, and reversion rate.
- Vaccine trials in mice using homologous and heterologous S. suis challenge models.
Main Results:
- An avirulent, streptomycin-dependent mutant of S. suis type 1/2 was successfully produced and characterized.
- The mutant demonstrated stability with low reversion rates.
- Vaccination with the mutant provided complete protection against S. suis types 1 and 1/2, and partial protection against type 2.
Conclusions:
- The characterized avirulent S. suis Str-D mutant is a promising candidate for a live attenuated vaccine.
- This mutant offers broad-spectrum protection against key S. suis serotypes.
- Further research may lead to improved control strategies for streptococcosis in swine.