Related Experiment Video
Updated: Sep 29, 2026

Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
Published on: November 6, 2019
Sterol composition in polyene antibiotic-sensitive and resistant strains of Malassezia pachydermatis
Y Uchida1, S Onodera, T Nakade
1Department of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Abstract:
The membrane sterol compositions of ten strains of Malassezia pachydermatis were analysed by gas-liquid chromatography. Six of these were wild-type strains from clinical sources that were sensitive to polyene antibiotics, whereas the other four strains were resistant mutants that had arisen after treatment with N-methyl-N'-nitrosoguanidine or UV radiation. The wild-type strains contained 838.8 to 1291.7 micrograms of sterol per gram of freeze-dried mycelium. The sterols were composed of 79% ergosterol, 19% fecosterol and 2% lichesterol. The mutant strains contained 277.1 to 348.8 micrograms/g of sterols composed of 76% ergosterol and 24% fecosterol.
Related Concept Videos
Biosynthesis of Lipids
The Skin Microbiota
Mechanism of Antibiotic Resistance in MRSA
Candidiasis
Antifungal Agents
Clinical Significance of Antibiotic Resistance

