Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD3- and CD28-activating pathways in HCV infection

J A Corado1, F I Toro, M L Baroja

  • 1Instituto de Inmunologia, Facultad de Medicina, Universidad Central de Venezuela, Sabana Grande, Caracas.

Viral Immunology
|January 1, 1994
PubMed
Summary

Hepatitis C virus (HCV) infection preserves T-cell activation pathways. Peripheral blood mononuclear cells (PBMC) from HCV patients showed normal proliferative responses and interleukin-2 receptor (IL-2R) expression upon stimulation, indicating intact CD3 and CD28 signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical immunology in Venezuela.

Immunology today·2014
Same author

Infusion of mesenchymal stem cells and rapamycin synergize to attenuate alloimmune responses and promote cardiac allograft tolerance.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2009
Same author

Receptor-interacting protein 2 is a marker for resolution of peritoneal dialysis-associated peritonitis.

Kidney international·2007
Same author

Opsonization by anti-dsDNA antibodies of apoptotic cells in systemic lupus erythematosus.

Autoimmunity·2007
Same author

HCV RNA sequences in eosinophils of chronic HCV-infected patients.

Journal of medicine·2007
Same author

Further description of early clinically silent lupus nephritis.

Lupus·2007

Area of Science:

  • Immunology
  • Virology
  • Cellular Biology

Background:

  • Chronic Hepatitis C Virus (HCV) infection can impact immune cell function.
  • Understanding T-cell responses in HCV is crucial for managing chronic liver disease.
  • Previous studies suggest potential immune dysregulation in HCV patients.

Purpose of the Study:

  • To investigate the proliferative capacity of peripheral blood mononuclear cells (PBMC) in HCV patients.
  • To assess the expression of interleukin-2 receptor (IL-2R) on PBMC stimulated via CD3 and CD28 pathways.
  • To compare immune responses between HCV patients with detectable viremia and healthy controls.

Main Methods:

  • Peripheral blood mononuclear cells (PBMC) were isolated from HCV-infected patients and healthy controls.

Related Experiment Videos

  • PBMC were stimulated with anti-CD3 monoclonal antibody (mAb) alone or in combination with anti-CD28 mAb.
  • Proliferative responses were measured, and IL-2R expression was analyzed using flow cytometry.
  • HCV RNA in PBMC was detected using nested PCR.

    • Main Results:

    • HCV patients and controls exhibited similar proliferative responses to anti-CD3 and/or anti-CD28 mAb stimulation.
    • No significant differences in proliferation were observed between HCV patients with detectable or undetectable HCV RNA in PBMC.
    • Both anti-CD3 and anti-CD3/anti-CD28 stimulation induced a significant increase in IL-2R expression in PBMC from both groups.
    • Basal CD28 expression levels were comparable between HCV patients and controls.

    Conclusions:

    • T-cell activation via CD3 and CD28 signaling pathways appears to be preserved in HCV-infected patients with chronic active liver disease.
    • The findings suggest that the fundamental mechanisms of T-cell activation remain intact despite chronic HCV infection.
    • This preservation of immune pathways may have implications for understanding disease progression and potential therapeutic strategies.