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Sequence patterns and hybridization analysis of clones generated by Alu-PCR
R Müllenbach1, S Tisborn, N Blin
1Institute of Anthropology and Human Genetics, University of Tübingen, Germany.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1994
Summary
Researchers analyzed Alu-PCR library clones, finding short PCR products and homologous regions limit filter hybridization and restriction length polymorphisms. However, short repeats offer potential variable sites for PCR analysis.
Area of Science:
- Molecular Biology
- Genomics
Background:
- Alu elements are repetitive sequences in the human genome.
- PCR-based methods are widely used for genetic analysis.
Purpose of the Study:
- To characterize clones from an Alu-PCR library.
- To assess the utility of these clones for genetic analysis.
Main Methods:
- Southern blot hybridization
- DNA sequencing
- Polymerase Chain Reaction (PCR)
Main Results:
- Clones exhibited short PCR product lengths.
- Homologous regions at both ends of probes limited filter hybridization.
- A low probability of restriction length polymorphisms was observed.
- Short repeats at the 3' ends of Alu repeats were identified.
Conclusions:
- Alu-PCR derived probes have limitations for filter hybridization and RFLP analysis.
- The identified short repeats represent a valuable source for PCR-based variable site discovery.
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