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Nucleotide sequence of ovine thioredoxin cDNA
L Droogmans1, Y Cleuter, E E Wollman
1Laboratoire de Chimie Biologique, Université Libre de Bruxelles, Rhode-St-Genèse, Belgium.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1994
Summary
Researchers cloned ovine thioredoxin cDNA from a bovine leukemia virus-infected cell line. This ovine thioredoxin protein shows high sequence identity to human and mouse thioredoxin, aiding comparative studies.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Thioredoxin is a crucial protein involved in redox homeostasis.
- Understanding thioredoxin's role across species can reveal conserved functions.
- Bovine leukemia virus (BLV) infection in cattle provides a model for studying host-pathogen interactions.
Purpose of the Study:
- To clone and characterize the ovine thioredoxin cDNA.
- To compare the ovine thioredoxin sequence with homologous proteins from other species.
- To provide a resource for further functional studies of ovine thioredoxin.
Main Methods:
- Construction of a lambda gt11 cDNA library from FLK cells (BLV-infected).
- Screening and isolation of the ovine thioredoxin cDNA clone.
- Sequence analysis and comparison with human and mouse thioredoxin.
Main Results:
- Successful cloning of a full-length ovine thioredoxin cDNA.
- The encoded protein consists of 105 amino acids.
- High sequence identity observed: 92% with human and 83% with mouse thioredoxin.
Conclusions:
- The cloned ovine thioredoxin represents a valuable tool for research.
- Conserved regions suggest similar functional roles across mammalian species.
- Further investigation into ovine thioredoxin's function in health and disease is warranted.