A SEM analysis of DMSO treated hydra polyps

Area of Science:

• Cell Biology
• Microscopy
• Biochemistry

Background:

• Dimethyl sulfoxide (DMSO) is commonly used for tissue permeabilization in biological research.
• Understanding the effects of DMSO on cell morphology is crucial for accurate experimental interpretation.

Purpose of the Study:

• To investigate the morphological effects of DMSO on hydra epithelial cells at concentrations used for tissue permeabilization.
• To assess the impact of DMSO on both cell surface and intracellular organization.

Main Methods:

• Scanning electron microscopy (SEM) was employed to visualize hydra polyps.
• Hydra polyps were exposed to DMSO at specific concentrations.
• Morphological changes in epithelial cells were analyzed using SEM.

Main Results:

• DMSO exposure caused significant changes in epithelial cell morphology, including cell rounding and the formation of large surface blebs.
• Numerous small projections appeared along the cell borders.
• Drastic intracellular organization changes were observed, but no evidence of cell junction or interstitial space opening was found.

Conclusions:

• DMSO significantly affects the morphology and intracellular organization of hydra epithelial cells.
• Caution is advised when interpreting cell behavior in tissues treated with DMSO due to these observed alterations.