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Updated: Oct 2, 2026

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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Use of metal-chelate affinity chromatography and hydrophobic interaction chromatography for purification of placental
1Hematological Scientific Center, Russian Academy of Medical Science, Moscow.
Journal of Chromatography. B, Biomedical Applications
|June 17, 1994
Abstract:
Placental protein 12 was isolated from amniotic fluid. Albumin was removed by means of ion-exchange chromatography on DEAE-Sepharose and chromatography on Blue-Sepharose. Complete purification was obtained by metal-chelate affinity chromatography and hydrophobic interaction chromatography under mild conditions for desorption. Using this procedure large quantities of a highly purified preparation can be obtained in one run.

