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Simple and sensitive assay of zonisamide in human serum by high-performance liquid chromatography using a solid-phase
1Department of Hospital Pharmacy, Okayama University Medical School, Japan.
Journal of Chromatography. B, Biomedical Applications
|June 17, 1994
Summary
A new method accurately measures zonisamide in serum using solid-phase extraction and HPLC. This rapid assay is suitable for pharmacokinetic studies, including in pediatric epilepsy patients.
Area of Science:
- Analytical Chemistry
- Clinical Chemistry
Background:
- Zonisamide is an antiepileptic drug requiring therapeutic drug monitoring.
- Accurate and sensitive serum assays are crucial for zonisamide pharmacokinetics.
Purpose of the Study:
- To develop and validate a rapid and sensitive method for zonisamide quantification in human serum.
Main Methods:
- Solid-phase extraction (SPE) using Bond-Elut cartridges for serum sample preparation.
- High-performance liquid chromatography (HPLC) with UV detection at 246 nm.
- Optimized mobile phase composition: acetonitrile-methanol-distilled water (17:20:63, v/v).
Main Results:
- The method demonstrated a low detection limit of 0.1 µg/ml in serum.
- High precision with within-day coefficients of variation of 4.2-5.6% and between-day of 5.1-9.1%.
Conclusions:
- The developed SPE-HPLC method is rapid, sensitive, and precise for zonisamide serum assay.
- This method is suitable for clinical pharmacokinetic studies of zonisamide, including in pediatric epilepsy patients.