High-performance liquid chromatography with UV detection and diode-array UV confirmation of isonicotinic acid hydrazide in cattle milk
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A Defilippi1, G Piancone, R Costa Laia
1Laboratorio di Sanità Pubblica USSL No. 40, Ivrea (TO), Italy.
Journal of Chromatography. B, Biomedical Applications
|June 17, 1994
Abstract:
A method for the determination of isonicotinic acid hydrazide (isoniazid) in milk was developed. Milk was deproteinized with trichloroacetic acid. Isoniazid was condensed with cinnamaldehyde and assayed on a reversed-phase HPLC system, with good sensitivity and accuracy (10 micrograms/l) with UV detection at 330 nm. Use of solid-phase extraction with a C18 cartridge allows the detection limit to be lowered to 0.1 microgram/l with UV detection and confirmation of isoniazid hydrazone from the diode-array UV spectrum.