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Related Experiment Videos

Atomic force microscopy of acid effects on dentin

G W Marshall1, M Balooch, R J Tench

  • 1Dept. of Restorative Dentistry, University of California, San Francisco.

Dental Materials : Official Publication of the Academy of Dental Materials
|July 1, 1993
PubMed
Summary

Atomic force microscopy revealed how acid treatment affects dentin surfaces. Early acid exposure causes linear peritubular depth changes and collagen scaffold collapse, crucial for understanding dentin bonding.

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Area of Science:

  • Biomaterials Science
  • Dental Materials Science
  • Surface Science

Background:

  • Acid etching is a critical step in dental restorative procedures.
  • Understanding dentin surface changes during acid treatment is vital for effective bonding.
  • Previous studies have not fully elucidated the dynamic morphological changes at the nanoscale.

Purpose of the Study:

  • To investigate the early-stage morphological and topographical changes of dentin surfaces upon acid treatment using Atomic Force Microscopy (AFM).
  • To quantify the rate of surface changes in peritubular and intertubular dentin.
  • To correlate observed changes with implications for dentin bonding agent penetration.

Main Methods:

  • Utilized Atomic Force Microscopy (AFM) to image highly polished dentin disks.

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  • Exposed dentin samples to 0.025 M nitric acid at 20-second intervals over 100 seconds.
  • Measured peritubular depth changes and intertubular surface movement.

    • Main Results:

    • Peritubular dentin depth changes were linear, measuring 0.005 microns/s.
    • Intertubular dentin initially moved at half the peritubular rate before plateauing due to collagen scaffold collapse.
    • No significant changes were observed in tubule center-to-center distances.

    Conclusions:

    • The differential surface dynamics between peritubular and intertubular dentin are critical for adhesive monomer penetration in dentin bonding.
    • Observed changes suggest partial collapse of the demineralized collagen matrix influences surface morphology.
    • AFM is a powerful tool for studying dentin conditioning and priming agents in dental applications.