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Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane
S Hitotsubashi1, Y Fujii, K Okamoto
1Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
FEMS Microbiology Letters
|October 1, 1994
Abstract:
The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophoresis and gel filtration showed that the molecular mass was 25 kDa.