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Angiotensinogen-deficient mice with hypotension

K Tanimoto¹, F Sugiyama, Y Goto

¹Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

The Journal of Biological Chemistry

|December 16, 1994

Summary

Mice lacking angiotensinogen, the precursor in the renin-angiotensin system, exhibit significantly lower blood pressure. This highlights the system's crucial role in regulating blood pressure.

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Area of Science:

Biochemistry
Physiology
Genetics

Background:

The renin-angiotensin system (RAS) is a critical hormonal cascade regulating blood pressure and fluid balance.
Angiotensinogen is the precursor to angiotensin I, which is converted to the potent vasoconstrictor angiotensin II.
The role of angiotensinogen in maintaining blood pressure in vivo is not fully understood.

Purpose of the Study:

To investigate the physiological role of angiotensinogen in blood pressure regulation.
To generate and characterize angiotensinogen-deficient mice.

Main Methods:

Homologous recombination was used to create angiotensinogen-deficient mice.
Systolic blood pressure was measured in homozygous mutant and wild-type mice.

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Main Results:

Angiotensinogen-deficient mice showed a complete absence of plasma angiotensin I.
Homozygous mutant mice exhibited significantly reduced systolic blood pressure (66.9 +/- 4.1 mm Hg) compared to wild-type controls (100.4 +/- 4.4 mm Hg).

Conclusions:

Angiotensinogen is essential for the production of angiotensin I and the overall function of the renin-angiotensin system.
The renin-angiotensin system plays an indispensable role in maintaining normal blood pressure.