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Related Experiment Videos

Biodegradable, effective substitute for xylene in the Ehrlich indole procedure

J M Miller1, M D Miller, P E Driscoll

  • 1Diagnostic Microbiology Section, Centers for Disease Control and Prevention, Atlanta, GA 30333.

Journal of Clinical Microbiology
|August 1, 1994
PubMed
Summary

Micro-Clear, a safer alternative to xylene, effectively detects indole in bacterial strains. This biodegradable reagent offers comparable results to traditional methods in the Ehrlich indole procedure.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics

Background:

  • The Ehrlich indole procedure is a key diagnostic test.
  • Xylene is the standard reagent but poses safety concerns.

Purpose of the Study:

  • To compare the efficacy of xylene, Hemo-De, and Micro-Clear in the Ehrlich indole procedure.
  • To evaluate Micro-Clear as a safer alternative to xylene.

Main Methods:

  • Tested 33 aerobic/facultative anaerobic and 50 strict anaerobic indole-positive bacterial strains.
  • Utilized xylene, Hemo-De, and Micro-Clear for indole extraction.
  • Compared indole detection rates across the three reagents.

Main Results:

  • Xylene detected indole in all tested isolates.

Related Experiment Videos

  • Micro-Clear detected indole in all aerobes and 49/50 anaerobes.
  • Hemo-De detected indole in 32/33 aerobes and 49/50 anaerobes.
  • No significant differences in detection rates were observed among reagents.

    • Conclusions:

    • Micro-Clear demonstrated comparable effectiveness to xylene for indole detection.
    • Micro-Clear is a safe, biodegradable, nonflammable, noncarcinogenic, and odorless alternative.
    • Micro-Clear should be considered a viable substitute for xylene in the Ehrlich indole procedure.