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Related Experiment Videos

CD43 (S7) expression identifies peripheral B cell subsets

S M Wells1, A B Kantor, A M Stall

  • 1Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, NY 10032-3702.

Journal of Immunology (Baltimore, Md. : 1950)
|December 15, 1994
PubMed
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CD43 (leukosialin) is expressed on most splenic B-1 cells, distinguishing them from other B cell types. This finding aids in identifying and characterizing distinct B cell populations in mice.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD43 (leukosialin) expression was previously noted on developing B cells and in vitro plasma cells, but not peripheral splenic B cells.
  • This study investigates the presence and role of CD43 on B cells in the spleen.

Purpose of the Study:

  • To determine the expression of CD43 on splenic B cell populations.
  • To differentiate B-1 cells from other B cell subsets using CD43 expression.

Main Methods:

  • Flow cytometric phenotyping of B cells from normal mice and radiation chimeras.
  • Utilized monoclonal antibody S7 to detect CD43 expression.
  • Analyzed expression of other cell surface markers including CD11b, heat stable antigen, B220, and B7.

Main Results:

Related Experiment Videos

  • CD43/S7 is expressed on over 90-95% of splenic B-1 cells and the majority of peritoneal B-1 cells.
  • CD43/S7 expression, along with other markers, effectively distinguishes B-1 cells from follicular, marginal zone, and immature B cells.
  • Splenic and peritoneal B-1 cells share phenotypes, with differences in CD11b, CD43/S7, and heat stable antigen expression observed in some peritoneal B-1 cells.

    • Conclusions:

    • CD43 is a reliable marker for identifying splenic B-1 cells.
    • CD43 expression, combined with other antigens, provides a robust method for phenotyping distinct B cell subsets.
    • Differences in antigen expression highlight the heterogeneity within B-1 cell populations and their distinction from conventional B cells.