[Kinetics of cyclization, coupled with ligated oligonucleotide modules, as a means of studying DNA bends]

A theoretical study of cyclization accompanying multimerization of oligonucleotide blocks is presented. The primary binding due to the cohesive ends of the blocks is subject to subsequent enzyme-catalyzed covalent fixation. Conditions were found for determining the thermodynamic probabilities of circular DNA conformations from the experimentally measurable rates of cyclization. An exact analytical description for the dynamics of multimerization without cyclization and an approximate description for cyclization accompanying multimerization are given.