A method of correcting DNA ploidy measurements in tissue sections

A new method of performing DNA ploidy measurements in tissue sections using image cytometry is described. The method involves an image processing "object" filtering operation to remove cut and overlapping nuclei, and DNA correction of larger nuclei for the part of the DNA cut away. The methodology of the technique is developed in detail, and the results of testing using sections of rat liver are presented. The results indicated reliable correction of DNA ploidy histograms to reconstruct the polyploid nature of this material. The sensitivity of the material to sectioning thickness errors and methods to overcome this were shown, along with an example of its use on prostate bioptic gun tissue sections.