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Related Experiment Videos

Group A streptococci efficiently invade human respiratory epithelial cells

D LaPenta1, C Rubens, E Chi

  • 1Department of Microbiology, University of Minnesota, Minneapolis 55455.

Proceedings of the National Academy of Sciences of the United States of America
|December 6, 1994
PubMed
Summary

Group A Streptococcus can invade human cells, leading to invasive infections like sepsis. Stationary-phase bacteria are efficiently internalized, with certain strains showing higher invasion capabilities than others.

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Area of Science:

  • Microbiology
  • Pathogenesis
  • Infectious Diseases

Background:

  • Group A Streptococcus (GAS) infections typically cause extracellular mucosal disease.
  • GAS is increasingly recognized as a cause of severe, invasive infections, including sepsis and shock.
  • Understanding GAS mechanisms for breaching mucosal barriers is crucial.

Purpose of the Study:

  • To investigate the capacity of Group A Streptococcus to internalize into human cells.
  • To identify bacterial growth phases and strains with enhanced internalization potential.
  • To compare GAS internalization frequencies with other known invasive bacteria.

Main Methods:

  • Development of an internalization assay using antibiotic resistance (penicillin and gentamicin).
  • Culturing human cells with different phases of Group A Streptococcus.

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  • Utilizing electron microscopy to visualize intracellular bacteria within vacuoles.
  • Comparing internalization frequencies across various GAS strains.

    • Main Results:

    • Stationary-phase Group A Streptococcus demonstrated efficient internalization into cultured human cells.
    • Internalized streptococci were observed within intracellular vacuoles.
    • Significant strain-dependent differences in internalization capacity were identified.
    • Type M1 GAS strains from blood infections showed high internalization frequencies, comparable to Salmonella and Listeria.

    Conclusions:

    • Group A Streptococcus possesses the ability to actively internalize into host cells.
    • Bacterial growth phase and strain type significantly influence internalization efficiency.
    • Certain GAS strains exhibit invasive potential comparable to well-established intracellular pathogens.