Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IS1032 from Acetobacter xylinum, a new mobile insertion sequence

T Iversen1, R Standal, T Pedersen

  • 1UNIGEN Center for Molecular Biology, University of Trondheim, Norway.

Plasmid
|July 1, 1994
PubMed
Summary

A new insertion sequence, IS1032, related to IS1031, was identified in Acetobacter xylinum. This mobile genetic element IS1032 shows transposition activity and shares similarities with other bacterial insertion sequences.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole blood long-chain n-3 fatty acids as a measure of fish oil compliance in children with acute lymphoblastic leukemia: a pilot study.

Prostaglandins, leukotrienes, and essential fatty acids·2022
Same author

PDRMIP: A Precipitation Driver and Response Model Intercomparison Project, Protocol and preliminary results.

Bulletin of the American Meteorological Society·2020
Same author

A PDRMIP multi-model study on the impacts of regional aerosol forcings on global and regional precipitation.

Journal of climate·2020
Same author

The Enterococcus Cassette Chromosome, a Genomic Variation Enabler in Enterococci.

mSphere·2018
Same author

Effect on non-vascular outcomes of lowering LDL cholesterol in patients with chronic kidney disease: results from the Study of Heart and Renal Protection.

BMC nephrology·2017
Same author

30-Day, 90-day and 1-year mortality after emergency colonic surgery.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2016

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Molecular Biology

Background:

  • Insertion sequences (IS) are mobile genetic elements found in bacteria.
  • IS1031 elements form a family of related IS in Acetobacter xylinum.
  • Understanding IS elements is crucial for studying bacterial genome dynamics.

Purpose of the Study:

  • To isolate and characterize a novel IS element in Acetobacter xylinum.
  • To determine the relationship of the new IS element to the IS1031 family.
  • To investigate the transposition activity and genomic distribution of the new IS element.

Main Methods:

  • Isolation of a new IS element (IS1032) from Acetobacter xylinum ATCC 23770.
  • Southern hybridization to detect IS1032-like sequences in various bacterial strains.

Related Experiment Videos

  • Analysis of insertion site duplication and terminal inverted repeats (IR).
  • Sequence analysis and comparison of the putative transposase with known IS elements.

    • Main Results:

    • IS1032 was isolated and characterized as a 916 bp element with imperfect terminal inverted repeats.
    • IS1032-like sequences were found in most examined Acetobacter xylinum strains and one Acetobacter aceti strain.
    • Transposition of IS1032 was confirmed by its insertion into a spontaneous mutant.
    • IS1032 encodes a putative transposase sharing 58% identity with IS1031 transposase and exhibits similarity to other IS transposases.

    Conclusions:

    • IS1032 is a novel member of the IS1031 family of insertion sequences in Acetobacter xylinum.
    • IS1032 is a mobile genetic element capable of transposition within the bacterial genome.
    • The findings highlight the diversity and relatedness of IS elements across different bacterial species.