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Transformation of Mollicutes with single-stranded Tn4001 DNA
P A Kapke1, K L Knudtson, F C Minion
1Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
Plasmid
|July 1, 1994
Abstract:
Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA.