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The changes in the myocardium under experimental conditions
1Institute of Histology and Embryology, 1st Medical Faculty, Charles University, Prague.
Sbornik Lekarsky
|January 1, 1993
Summary
Cyclophosphamide and isoprenaline alter myocardial cell structure similarly to cardiac surgery hypoxia. These interventions affect mitochondria and sarcoplasm, impacting cell permeability and ion transport.
Area of Science:
- Cardiovascular Biology
- Cellular Ultrastructure
- Biomedical Engineering
Background:
- Cardiac surgery and certain drug interventions can induce myocardial damage.
- Understanding the cellular mechanisms of this damage is crucial for developing protective strategies.
Purpose of the Study:
- To investigate the electron microscopic and morphometric effects of cyclophosphamide and isoprenaline on myocardium.
- To compare these effects with those observed during hypoxic periods of cardiac surgery.
Main Methods:
- Electron microscopy was used to examine myocardial tissue structure.
- Morphometric analysis, including computer-mediated image analysis, quantified cellular changes.
- Experimental animals were treated with cyclophosphamide and isoprenaline; human myocardium samples from surgery were also analyzed.
Main Results:
- Cyclophosphamide and isoprenaline induced significant changes in mitochondrial partial volume, comparable to hypoxic cardiac surgery.
- Sarcoplasm and mitochondria were identified as primary affected structures, with prompt proportion changes.
- Inhibition of Na, K-ATP-ases and altered F0 F1-ATP-ases in mitochondria are implicated in altered membrane permeability.
Conclusions:
- Drug-induced myocardial alterations share similarities with surgical hypoxia at the ultrastructural level.
- Morphometric evaluation provides statistically significant data across different experimental and clinical groups.
- Computer-aided image analysis validates manual methods under specific conditions for morphometric evaluation.