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Transfection of Escherichia coli by Mu DNA
Molecular & General Genetics : MGG
|December 22, 1976
Abstract:
Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.
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