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[In vitro study about the influence of recombinant interleukin-8 on human hematopoiesis]
1Zakładu Immunologii CSK WAM.
The influence of the recombinant human Interleukin-8 (IL-8) on in vitro clonogenicity of human bone marrow progenitors from the different haemopoietic lineages has been evaluated. Two different forms of IL-8 were used in this study: the longer one of the endothelial and shorter one of the monocytic origin. The bone marrow cells before the plating were enriched in CD34+ cells. We did not confirm that endothelial derived IL-8 inhibits the CFU-GM, BFU-E and CFU-Meg colony formation. Similarly monocytic IL-8 did nor inhibit neither the CFU-GM not the BFU-E colony growth. Nevertheless, small inhibitory effect of monocytic derived IL-8 have been found on the CFU-Meg colony formation.
The influence of the recombinant human Interleukin-8 (IL-8) on in vitro clonogenicity of human bone marrow progenitors from the different haemopoietic lineages has been evaluated. Two different forms of IL-8 were used in this study: the longer one of the endothelial and shorter one of the monocytic origin. The bone marrow cells before the plating were enriched in CD34+ cells. We did not confirm that endothelial derived IL-8 inhibits the CFU-GM, BFU-E and CFU-Meg colony formation. Similarly monocytic IL-8 did nor inhibit neither the CFU-GM not the BFU-E colony growth. Nevertheless, small inhibitory effect of monocytic derived IL-8 have been found on the CFU-Meg colony formation.