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Related Experiment Videos

Bioaerosol sampling in field studies: can samples be express mailed?

P S Thorne1, J L Lange, P Bloebaum

  • 1Department of Preventive Medicine and Environmental Health, University of Iowa, Iowa City.

American Industrial Hygiene Association Journal
|November 1, 1994
PubMed
Summary

Shipping bioaerosol samples from dairy barns affects microorganism concentrations, with different impacts depending on the sampling method and season. Careful consideration of storage and shipping is crucial for accurate microbial analysis.

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Area of Science:

  • Environmental microbiology
  • Agricultural science
  • Bioaerosol research

Background:

  • Bioaerosol sampling is vital for assessing microbial exposure in agricultural environments.
  • Understanding the impact of sample handling on microbial viability is critical for accurate data.

Purpose of the Study:

  • To evaluate the effects of sample storage and shipping on viable microorganism concentrations in dairy barn bioaerosols.
  • To compare the performance of three different bioaerosol sampling methods: all-glass impinger (AGI) with peptone, AGI with betaine, and nuclepore filtration and elution (NFE).

Main Methods:

  • Conducted bioaerosol sampling in 25 dairy barns during summer and winter.
  • Utilized duplicate, simultaneous, side-by-side sampling with AGI (peptone/betaine) and NFE methods.

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  • Analyzed one sample immediately and express-mailed the duplicate to assess shipping effects.

    • Main Results:

    • AGI peptone method showed no change in winter but increased mesophilic bacteria in summer after shipping.
    • AGI betaine showed increased mold concentrations after shipping in winter.
    • NFE samples had decreased yeasts and mesophilic bacteria after shipping.
    • Sampling methods showed no significant differences in winter, but seasonal variations in microbial concentrations were observed.

    Conclusions:

    • Shipping significantly impacts viable bioaerosol concentrations, varying by microorganism type, sampling method, and season.
    • The choice of sampling method and adherence to prompt analysis are crucial for reliable dairy barn bioaerosol monitoring.
    • Further research is needed to optimize sampling and shipping protocols for accurate microbial assessments.